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Goal-oriented Script Generation is a new task of generating a list of steps that can fulfill the given goal. In this paper, we propose to extend the task from the perspective of cognitive theory. Instead of a simple flat structure, the steps are typically organized hierarchically — Human often decompose a complex task into subgoals, where each subgoal can be further decomposed into steps. To establish the benchmark, we contribute a new dataset, propose several baseline methods, and set up evaluation metrics. Both automatic and human evaluation verify the high-quality of dataset, as well as the effectiveness of incorporating subgoals into hierarchical script generation. Furthermore, We also design and evaluate the model to discover subgoal, and find that it is a bit more difficult to decompose the goals than summarizing from segmented steps. 

User preferences are usually dynamic in real-world recommender systems, and a user’s historical behavior records may not be equally important when predicting his/her future interests. Existing recommendation algorithms – including both shallow and deep approaches – usually embed a user’s historical records into a single latent vector/representation, which may have lost the per item- or feature-level correlations between a user’s historical records and future interests. In this paper, we aim to express, store, and manipulate users’ historical records in a more explicit, dynamic, and effective manner. To do so, we introduce the memory mechanism to recommender systems. Specifically, we design a memory-augmented neural network (MANN) integrated with the insights of collaborative filtering for recommendation. By leveraging the external memory matrix in MANN, we store and update users’ historical records explicitly, which enhances the expressiveness of the model. We further adapt our framework to both item- and feature-level versions, and design the corresponding memory reading/writing operations according to the nature of personalized recommendation scenarios. Compared with state-of-the-art methods that consider users’ sequential behavior for recommendation, e.g., sequential recommenders with recurrent neural networks (RNN) or Markov chains, our method achieves significantly and consistently better performance on four real-world datasets. Moreover, experimental analyses show that our method is able to extract the intuitive patterns of how users’ future actions are affected by previous behaviors. 

Optical density (OD) is widely used to estimate the density of cells in liquid culture, but cannot be compared between instruments without a standardized calibration protocol and is challenging to relate to actual cell count. We address this with an interlaboratory study comparing three simple, low-cost, and highly accessible OD calibration protocols across 244 laboratories, applied to eight strains of constitutive GFP-expressingE. coli. Based on our results, we recommend calibrating OD to estimated cell count using serial dilution of silica microspheres, which produces highly precise calibration (95.5% of residuals  <1.2-fold), is easily assessed for quality control, also assesses instrument effective linear range, and can be combined with fluorescence calibration to obtain units of Molecules of Equivalent Fluorescein (MEFL) per cell, allowing direct comparison and data fusion with flow cytometry measurements: in our study, fluorescence per cell measurements showed only a 1.07-fold mean difference between plate reader and flow cytometry data.